FIGURE

Fig. 3

ID

ZDB-FIG-130212-13

Publication

Coffey et al., 2013 - Novel oxytocin gene expression in the hindbrain is induced by alcohol exposure: transgenic zebrafish enable visualization of sensitive neurons

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Fig. 3

Forebrain and hindbrain pax2a:GFP positive cells are sensitive to ethanol exposure.

A, the duration and embryonic stage of 2% ethanol treatments illustrated with colored bars for Tg(pax2a:GFP) embryos, as described in Fig. 1. B-E, ventral views (B, D) and dorsal views (C, E) of Tg(pax2a:GFP) embryos at 52 hpf. B, C, are controls. D, E, were treated with 2% ethanol for 24 hrs at the 14 somite stage. B, D, a small, distinct group of neurons in the forebrain (arrows in B) were missing after ethanol exposure (D). The location of the eyes is outlined (dashed white lines). C, E, neurons around the midbrain-hindbrain region were mostly unaffected, hindbrain neurons were drastically reduced (arrowheads), and spinal cord neurons appeared slightly reduced. Tectum (te).

Expression Data

Gene:	EGFP
Fish:	e1Tg
Condition:	chemical treatment: ethanol
Anatomical Terms:	forebrain neuron hindbrain neuron midbrain hindbrain boundary spinal cord neuron
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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