FIGURE

Fig. 2

ID

ZDB-FIG-130128-14

Publication

Campbell et al., 2012 - Two types of tet-on transgenic lines for doxycycline-inducible gene expression in zebrafish rod photoreceptors and a gateway-based tet-on toolkit

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Fig. 2

Time course of doxycycline-induced GFP expression in Tg(Xla.rho:rtTA, TRE:GFP) larvae.

Confocal z-projections of retinal sections from 6 dpf Tg(Xla.rho:rtTA, TRE:GFP); alb^/ larvae that were (A) untreated or treated with doxycycline (Dox) for (B) 1, (C) 2, (D) 4, (E) 8, (F) 16, (G) 24, (H) 48, or (I) 72 h. Anti-Rhodopsin immunofluorescence (red) labels rod photoreceptor outer segments and is visible for all treatment conditions. Anti-GFP immunofluorescence (green) is not visible in the untreated retinal section (A). The number of rods showing anti-GFP immunofluorescence (green) noticeably increases with an increase in the Dox exposure time (B–I). Scale bar (A), 50 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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