FIGURE

Fig. 1

ID

ZDB-FIG-121212-13

Publication

Pase et al., 2012 - Neutrophil-Delivered Myeloperoxidase Dampens the Hydrogen Peroxide Burst after Tissue Wounding in Zebrafish

Other Figures

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Fig. 1

Leukocytes Are Required for the Initial Decline in Wound Margin H₂O₂ Concentrations(A) (i) Mean HyPer ratios reflecting the wound zone [H₂O₂] profile of WT (injured and uninjured), cloche mutant (clo) and spi1/csf3r morphant embryos. Note the significantly sustained, elevated HyPer ratio/[H₂O₂] in leukocyte-deficient clo and spi1/csf3r morphants. Insets (ii, iii) show the individual median HyPer data and p values (natural permutation test). The n values are as follows (embryos/independent days/scans): injured WT, 5/2/4; spi1/csf3r morphants, 4/2/3; clo 6/3/5; uninjured WT, 5/1/5.(B) HyPer heat maps depicting [H₂O₂] across the wounded tail fin at five sequential time points. Accompanying overlaid transmission (Trans) and DsRED2 images demonstrate number and position of Tg(lyz:DsRED2) neutrophils. Note the single DsRED2-positive cell in the spi1/csf3r morphant (black arrow head). clo did not carry the lyz:DsRED2 transgene. Scale bar (applies to all panels) represents 200 μm. Heat map legend applies to all figures. The stills are from Movie S1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-spi1b + MO3-csf3r Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39
Knockdown Reagents:	MO1-spi1b MO3-csf3r
Observed In:	regulation of hydrogen peroxide metabolic process
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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