Fig. 2

Marker analysis of msgn1;spt double-deficient embryos. Wild-type embryos injected with 5mis MO (A, E, I, and M) or msgn1 MO (B, F, J, and N) and spt homozygous embryos injected with 5mis MO (C, G, K, and O) or msgn1 MO (D, H, L, and P) were fixed at the 24-somite stage and hybridized with tbx24 (A-D), papc (E-H), ntl (I-L) or wnt8 (M-P) probes. The expression of tbx24 in the PSM is severely reduced in the msgn1 MO-injected spt homozygous embryo (D) compared with that in the wild-type (A), msgn1 MO-injected wild-type (B) or spt single-mutant embryo (C). Similarly, papc-positive migrating cells in the anterior PSM (indicated by arrowheads) were not detected in the msgn1 MO-injected spt homozygous embryo (H). Injection of msgn1 MO into wild-type embryos increased the expression area of ntl and wnt8 in the tailbud (J and N) compared with that for the control MO (I and M). In addition, ntl and wnt8-expressing cells are also increased by msgn1 MO in spt mutant embryos (K, L, O, and P).