FIGURE

Fig. 1

ID

ZDB-FIG-121023-1

Publication

Wang et al., 2012 - Wnt signaling regulates postembryonic hypothalamic progenitor differentiation

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Fig. 1

Identification of Wnt-Responsive Cells in the Zebrafish Hypothalamus (A) Ventral view of TCFSiam:GFP. (B) Costaining with PCNA, Sox3, and Hu. Blue ovals label the presumptive lateral recess, and red ovals label the presumptive posterior recess. White box in (A) marks the area shown. (C) Maximum intensity confocal Z-projection of TCFSiam:GFP at 4 dpf. In nuclear stained ventral view of brain on left, white box marks hypothalamic area shown on right. Red oval marks the posterior recess. (D) Costaining of TCFSiam:GFP with PCNA, Sox3, and HuC/D. Yellow box in (C) marks the area shown. (E) Costaining of TCFSiam:GFP with dlx5/6:gfp and serotonin. Yellow box in (C) marks the area shown. (F) Dissecting microscope ventral and sagittal views of TCFSiam:GFP in the adult hypothalamus. Red oval labels the presumptive posterior recess. (G) Costaining of TCFSiam:GFP with Sox3, Hu, 5HT, dlx5/6:mCherry, and GABA in the adult posterior hypothalamus. Yellow box in (F) marks the area shown. Single optical sections from ventral views are shown in all panels, unless otherwise indicated. Small orange circles label cells with colocalization, and small magenta circles label cells without colocalization. Scale bars represent 80 μm (A–E,G), and 250 μm (F). See also Figure S1.

Expression Data

Genes:	GFP sox3
Antibodies:	Ab1-elavl Ab1-GABA Ab2-serotonin
Fish:	ia4Tg
Anatomical Terms:	GABAergic neuron hypothalamus hypothalamus multi fate stem cell hypothalamus neuron hypothalamus neuronal stem cell lateral recess neuroblast (sensu Vertebrata) neuronal stem cell posterior recess serotonergic neuron
Stage Range:	Prim-15 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 23(3), Wang, X., Kopinke, D., Lin, J., McPherson, A.D., Duncan, R.N., Otsuna, H., Moro, E., Hoshijima, K., Grunwald, D.J., Argenton, F., Chien, C.B., Murtaugh, L.C., and Dorsky, R.I., Wnt signaling regulates postembryonic hypothalamic progenitor differentiation, 624-636, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell