Fig. 3

Non-neuronal cells associated with the dorsal root ganglia (DRG) proliferate. (A) Confocal projection of a ganglion at five dpf, showing sensory neurons labeled with Elavl (green) and an associated cell labeled with pH3 (red; blue arrow). Nuclei were labeled with DAPI (blue). (B-C) Ganglia of larvae at five dpf following BrdU incorporation (red). Neurons are labeled with Elavl (red) and nuclei are labeled with DAPI (blue). (B) Following a BrdU pulse at five dpf, cells associated with the DRG incorporate BrdU (yellow arrowhead), but neurons do not. (C) In a larva that was pulsed with BrdU at two dpf and chased to five dpf, a BrdU + neuron is located in the DRG (white arrowhead). (D) A ganglion in a 25 dpf, 6.25 mm larval fish exposed to a pulse of BrdU at 25 dpf, shows BrdU + cells (red; yellow arrowhead) and pH3 labeled cells (blue; blue arrow) are intermingled among Elavl + neurons (green), no neurons are labeled with BrdU or pH3. Dashed lines indicate levels of XZ and YX insets. Scale bars, 20 μm. BrdU, bromodeoxyuridine; DAPI, 4′,6-diamidino-2-phenylindole; dpf, days postfertilization. pH3, phosphohistone H3.