FIGURE

Fig. 7

ID

ZDB-FIG-121004-22

Publication

An et al., 2012 - The zebrafish sf3b1(b460) mutant reveals differential requirements for the sf3b1 pre-mRNA processing gene during neural crest development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Neural crest progenitor and cranial ganglia phenotypes of sf3b1^b460 mutants. There is reduced melanoblast expression of mitfa (A,B) and nearly absent melanoblast expression of dct (C,D) in sf3b1^b460 mutants. (E-J) Pharyngeal arches are dramatically reduced in sf3b1^b460 mutant embryos. (E-H) Lateral view of whole-mount in situ embryos with dlx2 antisense RNA probe, arch progenitors denoted by arrows. (I,J) Dorsal view of flat-mounted in situ embryos with dhand antisense RNA probe, pharyngeal arches 1 and 2 are decreased and 3, 4 and 5 are absent in sf3b1^b460 mutants. (K-N) the cranial ganglia are also affected in sf3b1^b460 mutants. (K,L) Whole-mount antibody staining with zn-12. Arrows in (K,L) show the trigeminal ganglia. (M,N) Whole-mount antibody staining with anti-acetylated tubulin antibody. Arrows in (M,N) show the decreased and disorganized trigeminal ganglia in sf3b1^b460 mutants compared to wildtype siblings. (O,P) Crestin expressing cells are present in sf3b1^b460 mutant embryos. However, these cells are reduced in numbers, particularly in the cranial region and fail to migrate normally in the sf3b1^b460 mutant (P) compared to wildtype embryos (O).

Expression Data

Genes:	crestin dct dlx2a hand2 mitfa
Antibody:	zn-12
Fish:	AB sf3b1^b460/b460
Anatomical Terms:	melanoblast neural crest cell pharyngeal arch 1 pharyngeal arch 2 pharyngeal arch 3 pharyngeal arch 4 pharyngeal arch 5 trigeminal placode trunk melanoblast trunk neural crest cell
Stage Range:	14-19 somites to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sf3b1^b460/b460
Observed In:	melanocyte melanocyte differentiation pharyngeal arch trigeminal ganglion trigeminal ganglion neuron
Stage Range:	14-19 somites to Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Dev. Biol.