FIGURE

Fig. 1

ID

ZDB-FIG-120920-2

Publication

Jensen et al., 2012 - Opposing Effects of Circadian Clock Genes Bmal1 and Period2 in Regulation of VEGF-Dependent Angiogenesis in Developing Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Constant Light Exposure Inhibits Developmental AngiogenesisTg(Fli1:EGFP)^y1 transgenic zebrafish embryos exposed to LD (A–D), LL (A–D), or DD (C and D) were examined for ISV development at 24 hpf (C and D) or for SIV development at 72 hpf (C and D) using confocal microscopy.(A) Morphology of zebrafish embryos was examined at 24 hpf and 48 hpf under LD and LL conditions.(B) Average ± SEM somite lengths and head angles were quantified under LD and LL conditions (n = 7–9/group).(C) Zebrafish embryos were raised under LD, DD, or LL conditions from 1 hpf. Dashed lines indicate the maximal length of ISVs. Yellow dashed lines encircle the SIV. Scale bar for ISV is 50 μm and for SIV is 100 μm.(D) Quantification of the average ± SEM. ISV length (n = 52–54 embryos/group) of 24 hpf embryos and the SIV area of 72 hpf embryos (n = 48 embryos).(E) RT-PCR analysis of average zebrafish period2 (P2) expression ± SEM under LD, LL, and DD conditions (a pooled sample of 25 zebrafish embryos/group).(F) Densitometric quantification of average period2 expressions shown in (E).Bars in (A) = 500 μm. S, somite; NC, notochord; H, hindbrain; M, mesencephalon; E, eye. Blue lines in (A) indicate the head angle. p < 0.05 and p < 0.01. NS, not significant.See also Figure S2.

Expression Data

Gene:	EGFP
Fish:	y1Tg
Condition:	constant light
Anatomical Terms:	intersegmental vessel subintestinal vein
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg
Condition:	constant light
Observed In:	intersegmental vessel subintestinal vein
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.