Fig. 6

Defective hindbrain angiogenesis in integrin α_v and β₈ double morphants. (A) At 48 hpf, a lateral view of the hindbrain of the wild type embryo shows central arteries (CtA, red arrows) sprouting from posterior communicating segment (PCS) and basilar artery (BA) and draining into primordial hindbrain channel (PHBC) (B) At 72 hpf, the CtAs penetrate deeply into the hindbrain in wild type embryos (red double-headed arrow). (C) 48 hpf integrin α_vβ₈ double morphants show CtAs with reduced number and atypical growth direction (yellow arrows). (D) At 72 hpf, the CtAs in integrin α_vβ₈ morphants invade into the hindbrain matter, but have reduced length as compared to wild type embryos (yellow double-headed arrow). (E) In a dorsal view regular, lumenized CtA are observed in 48 hpf wild type embryos. (F) At 72 hpf, the basic network structure of the CtA remains similar, although is more refined. (G) In contrast, in 48 hpf integrin α_vβ₈ double morphants there is a reduced number of CtAs, with very irregular morphology (yellow arrowhead). (H) At 72 hpf, the structure of CtA vasculature in α_vβ₈ double morphants has an irregular, plexus-like morphology. (I) Quantitation of the percentage normal CtAs at 50, 74 and 98 hpf in wild type embryos (wt) and integrin α_vβ₈ double morphants (dMO).