Fig. 1
- ID
- ZDB-FIG-120815-41
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- Witzel et al., 2012 - The LIM Protein Ajuba Restricts the Second Heart Field Progenitor Pool by Regulating Isl1 Activity
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Ajuba Interacts with Isl1 (A) FLAG-HA-tagged Ajuba and Isl1 were transiently expressed in HEK293T cells. Proteins were immunoprecipitated with an anti-HA antibody and detected by immunoblot with an anti-Isl1 antibody. IP, immunoprecipitation; WB, western blot. (B) Isl1 and Myc-tagged LPP, Zyxin, LIMD1, and WTIP were transiently expressed in HEK293T cells, and immunoprecipitation with an anti-Isl1 antibody followed by immunoblot analysis with an anti-myc antibody was performed. (C) Coimmunoprecipitation of extracts from day 5 EBs differentiated from ES cells using anti-Isl1 antibody and detected with anti-Ajuba antibody. (D) Schematic representation of wild-type Isl1 and Isl1 deletion constructs (top). FLAG-HA-tagged Ajuba and Isl1 or Isl1 deletion constructs were transiently expressed in HEK293T cells, and immunoprecipitation with an anti-Isl1 antibody followed by immunoblot analysis with an anti-HA antibody was performed (bottom). (E) Schematic representation of wild-type Ajuba and Ajuba deletion constructs (top). FLAG-HA-tagged Ajuba or Ajuba deletion constructs and Isl1 were transiently expressed in HEK293T cells, and immunoprecipitation with an anti-Isl1 antibody followed by an immunoblot analysis with an anti-HA antibody was performed. NES, nuclear export sequence. |
Reprinted from Developmental Cell, 23(1), Witzel, H.R., Jungblut, B., Choe, C.P., Crump, J.G., Braun, T., and Dobreva, G., The LIM Protein Ajuba Restricts the Second Heart Field Progenitor Pool by Regulating Isl1 Activity, 58-70, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell