Fig. S4

cey mutants have increased proliferation in the retina

Proliferation is expanded in cey mutant retinas. At 4 dpf there are more pH3+ (G2/M) cells in cey mutants (B) as compared to wild-type (A). In addition these cells are not confined to the peripheral CMZ region, but are throughout the central retina (white arrowheads; B). By 5 dpf the number of pH3+ cells is reduced and confined in cey larvae (D), more closely resembling wildtype larvae (C). Quantification shows that cey mutants have significantly more pH3+ cells than wild-type at 3, 4 and 5 dpf, although the number of cells decreases over time in both wild-type and cey mutants (E). Whole Z-stacks of dorsal view retinas were projected and the number of pH3+ cells were counted and then averaged to determine the average number of mitotic cells in wild-type and cey larvae at 3, 4 and 5 dpf (E, see methods for more details on quantification). cey mutants also have an increased number of cells in S-phase as compared to wild-type (F) indicating there are more proliferating cells at 3 dpf. The average number of BrdU positive cells in five lateral view sections was calculated for each retina (F, see methods for more details). Scale Bars are 130μm.