Fig. S4
- ID
- ZDB-FIG-120612-21
- Publication
- Lindeman et al., 2012 - Localized Products of futile cycle/ lrmp Promote Centrosome-Nucleus Attachment in the Zebrafish Zygote
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Western Blotting and Additional Protein/RNA Localization Analysis, Related to Figure 4 (A) Embryo lysates from wild-type and two fue mutant clutches at cleavage stages and 4 hpf were probed with anti-LrmpMD antibody. A band at 200 kDa (red arrow) likely corresponds to Lrmp and appeared reduced in mutant compared to wild-type, while a shorter 160 kDa band was present in mutant lysates. The observed 200 kDa molecular weight is greater than predicted for the zebrafish Lrmp isoforms (approximately 159 kDa - 162 kDa), however mouse and human Lrmp proteins also exhibit higher-than-expected molecular weights on denaturing gels (predicted at 62 kDa and 59 kDa, observed at 75 kDa and 69 kDa, respectively) [11,44]. The identity of the 25 kDa band seen in both mutant and wild-type is not known and unlike the 200 kDa species, was not recognized by the more C-terminal Lrmp antibody (data not shown). Wild-type 24 hpf lysates did not show any prominent signal. Anti-actin antibody was used as a loading control. |