FIGURE

Fig. 5

ID

ZDB-FIG-120604-4

Publication

Poulain et al., 2002 - Mezzo, a paired-like homeobox protein is an immediate target of Nodal signalling and regulates endoderm specification in zebrafish

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Fig. 5

mezzo, casanova, bon/mixer and ntl are immediate early targets of Nodal signalling, while sox17 requires post MBT protein synthesis. Embryos at the one- to four-cell stage were microinjected with 50 pg of TARAM-A* mRNA, treated continuously with 50 mg/ml of cycloheximide, starting at the 64- to 128-cell stage and analysed at dome-30% epiboly stage for the expression of sox17 (A-E), ntl (F-J), mezzo (K-O) and casanova (P-T). (A,F,K,P) Untreated and uninjected control embryos. (B,G,L,Q) Uninjected control embryos treated with cycloheximide. (C,H,M,R) Control untreated embryos injected with TARAM-A*. (D,E,I,J,N,O,S,T), Embryos injected with TARAM-A* and treated with cycloheximide. Although treatment with cycloheximide prevents induction of sox17, some ectopic mezzo, ntl, bon/mixer and cas expression is clearly visible in absence of protein synthesis.

Expression Data

Genes:	sebox sox17 sox32 tbxta
Fish:	AB
Condition:	chemical treatment: cycloheximide
Anatomical Terms:	margin margin dorsal region marginal blastomere dorsal side
Stage:	30%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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