FIGURE

Fig. 5

ID

ZDB-FIG-120525-20

Publication

Telfer et al., 2012 - neb: a zebrafish model of nemaline myopathy due to nebulin mutation

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Fig. 5

Nemaline bodies in neb skeletal muscle. (A) Ultrastructural analysis reveals the presence of aggregates similar to nemaline bodies. Top panel: a typical example of a nemaline body (NB) is depicted in a myofiber examined in cross-section. S, sarcomere; N, nucleus. Scale bar: 1 μm. Bottom panel: higher magnification of the area denoted by the white box demonstrates that the aggregates are composed primarily of filamentous material. Scale bar: 250 nm. (B) Immunohistochemical analysis of isolated myofibers from 3-dpf neb embryos using an antibody to α-actinin. Top panel: frequent areas of aberrant α-actinin accumulation were detected (arrow), consistent with the presence of nemaline bodies. Bottom panel: co-staining with phalloidin (red) reveals the presence of actin in the aggregated areas (arrow). White lines denote the sarcolemmal membrane.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	neb^{hu2849/hu2849} (AB)
Observed In:	skeletal muscle cell filamentous actin
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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