Fig. 5

Dissection of the Fugu CNE 6 lens enhancer. (A) Schematic drawing of CNE 6 to indicate the subdivision into three parts (ABC) for the deletion analysis and the position of the six putative Sox binding sites (red) numbered 1–6. (B) Percentage of GFP-positive lenses in zebrafish injected with various deletion clones of Fugu CNE 6 (ABC = full-length CNE 6). (C) Percentage of GFP-positive lenses in zebrafish injected with different mutated versions of CNE 6. All 6 putative Sox sites were mutated separately (soxm1-6). (D,E) Transient GFP expression in 52 hpf-old zebrafish injected with the Fugu CNE 6 WT sequence (D) or a variant carrying a mutation in putative Sox site 3 (E). The lens is indicated by an arrow. Note that absence of lens expression in (E) correlates with lower expression levels in telencephalon (te) and rhombencephalon (rho). Tr (Takifugu rubripes, Fugu).