FIGURE

Fig. S1

ID

ZDB-FIG-120405-13

Publication

Cadwalader et al., 2012 - 2-O-sulfotransferase regulates Wnt signaling, cell adhesion and cell cycle during zebrafish epiboly

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Fig. S1

Adhesion defects in 2-OST MO are cell autonomous. (A-F) Single planes of z-stack at 4 hpf of mosaic embryos formed from injecting either control MO+lissamine-conjugated MO (B,C) or 2-OST MO+lissamine-conjugated MO (E,F) into a single cell at the 32-cell stage. Anti-β-catenin for control MO mosaic (A) or 2-OST MO (D). (G-I) Animal caps removed and immediately imaged from control MO (G), 2-OST MO (H), or 2-OST MO+2-OST RNA (I) embryos to examine adhesion defects. Minimal cells were observed around control MO and rescue-injected animal caps, but many cells were observed around 2-OST MO, indicating decreased adhesion.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-hs2st1a AB + MO2-hs2st1a
Knockdown Reagents:	MO1-hs2st1a MO2-hs2st1a
Observed In:	cell-cell adhesion
Stage:	Sphere

Phenotype Detail

Acknowledgments

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