FIGURE

Fig. 1

ID
ZDB-FIG-120329-41
Publication
Gomez et al., 2012 - Identification of Vascular and Hematopoietic Genes Downstream of etsrp by Deep Sequencing in Zebrafish
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Fig. 1

Workflow diagram.

Embryos were either uninjected or injected with etsrp RNA at the one cell stage, then raised until the late gastrulation stages when the flk1-gfp transgenic reporter is induced ectopically. Equal paired groups of embryos were pooled for mRNA extraction, library construction, and solexa mRNA-sequencing. Samples were pre-validated prior to sequencing by RT-PCR with fli1A, the 32UTR of etsrp, as well as scl, which were all preferentially induced as expected by etsrp overexpression (e) relative to uninjected controls (u).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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