Fig. 4

Ddx46 expression in the developing zebrafish.

(A–K) Ddx46 expression was examined using whole-mount in situ hybridization in WT embryos or larvae at the 128-cell (A), 6-somite (B), 1-dpf (C), 2-dpf (D–G), and 4-dpf (H–K) stages. Lateral view, animal pole to the top (A). Lateral views, anterior to the left (B, C, D, F, H, J). Dorsal views, anterior to the top (E, G, I, K). The Ddx46 transcript was maternally supplied and continued to be expressed ubiquitously during the somitogenesis stages (A, B). By 1 dpf, Ddx46 expression became restricted to the head region (C). At 2 dpf, strong Ddx46 expression was prominent in the head, pectoral fin bud, and digestive organs (D–G). At 4 dpf, Ddx46 expression was further restricted to the retina, telencephalon, midbrain, midbrain-hindbrain boundary, branchial arch, esophagus, liver, pancreas, and intestinal bulb (H–K). No Ddx46 transcript was detected in the somite (arrowhead in H). (L, M) Ddx46 expression was examined using whole-mount in situ hybridization in WT larvae at 3 dpf. Dorsal views, anterior to the top (L). A transverse section was cut at the level indicated by the black dotted line in L. The section revealed Ddx46 expression in the intestine and exocrine pancreas, but not in the endocrine pancreas (M). b, branchial arches; e, esophagus; en, endocrine pancreas; ex, exocrine pancreas; i, intestine; ib, intestinal bulb; L, liver; m, midbrain; mhb, midbrain-hindbrain boundary; p, pancreas; pf, pectoral fin bud; r, retina; t, telencephalon.