Fig. 4

slc1a2b knockdown phenocopies tnt mutants at 48 and 96 hpf. Kinematic traces are shown, with 0° indicating a straight body and positive and negative angles representing body bends in opposite directions. Time is shown in seconds. Five representative traces are shown for each condition. (A) Wild-type larvae exhibit one large amplitude bend, a C-start, followed by smaller amplitude body bends. (B) tnt larvae exhibit several large amplitude body bends throughout the escape response. (C) Embryos injected with the standard control morpholino are similar to wild-type. (D) slc1a2b morphant larvae exhibit many large amplitude C-bends similar to tnt mutants. (E) Quantification of the number of large amplitude body bends (defined as greater than 110°) observed within an escape. Both tnt mutant and slc1a2b morphant larvae perform significantly more large amplitude body bends (n = 20, p < 0.05). Asterisks indicate significant differences. (F) tnt mutant and slc1a2b morphant larvae also performed significantly longer escape responses (n = 20, p < 0.05). (G) Embryos injected with the tnt morpholino also demonstrate body shortening along the rostral-caudal axis at 96 hpf, but not 48 hpf, compared to embryos injected with the control morpholino (n = 12 to 14, p < 0.01).