Fig. 1

miR-221 Is Required for Vascular Development (A) Whole-mount in situ hybridization of embryos at 48 hr postfertilization (hpf). Dorsal aorta and posterior cardinal vein are indicated by a bracket and A or V, respectively. Arrows indicate intersegmental blood vessels (ISV). Scale bar = 50 μm. (B) Left column, transmitted light images of embryos at 30 hpf injected with indicated MO. Scale bar = 250 μm. Right column, confocal micrographs of trunk vessels at 30 hpf in Tg(kdrl:egfp)^la116 embryos injected with indicated MO. ISVs indicated by arrows and DLAV by arrowheads. Scale bar = 50 μm. (C) Tg(kdrl:egfp)^la116 embryos at 27 hpf injected with 10 ng control or miR-221 MO. aa1, aortic arch 1; PHBC, primordial hindbrain channel; LDA, lateral dorsal aorta. Scale bar = 50 μm. (D) Tg(fli1a:egfp)^y1 embryos at 5 days postfertilization (dpf) injected as in (C). DA, dorsal aorta; PCV, posterior cardinal vein; position of thoracic duct is indicated by arrows. Scale bar = 25 μm. (E) Transmitted light images of larvae at 5 dpf injected as in (C). Scale bar = 500 μm. All panels are lateral views, dorsal is up, anterior to the left.