Fig. 7

Both Morpholino Knockdown of Pkd1a/b and Treatment with DAPT Result in Dorsal Axis Curvature in Zebrafish Embryos, which Can Be Rescued by Expression of the PC1-CTT (A) Morpholinos corresponding to the zebrafish Pkd1a and Pkd1b PC1 genes were injected into zebrafish embryos at the one- to two-cell stage to impair expression of the two Pkd1 genes. The embryos were subsequently injected with 300 nM of mRNA encoding HA-PC1-CTT, where indicated, and imaged at 3 days post fertilization (dpf). (B) Embryo phenotypes were scored based on the degree of dorsal tail curvature: mild (<90°), moderate (>90°), and severe (tail tip crossing the body axis). The data represent the averages of three separate experiments; error bars represent SEM. (C) Embryos were injected with 300 nM mRNA encoding either GFP (control) or HA-PC1-CTT at the one- to two-cell stage, then immersed in embryo media containing 25 μM DAPT, imaged at 3 dpf, and scored for the tail curvature phenotype.