FIGURE

Fig. 6

ID

ZDB-FIG-120206-39

Publication

Bedell et al., 2012 - The lineage-specific gene ponzr1 is essential for zebrafish pronephric and pharyngeal arch development

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Fig. 6

Loss of capillary blood flow in ponzr1 morphants. (A,C,E) Tg(wt1b:EGFP) in the podocytes was used to identify the glomerulus in a 3 dpf larvae. (B,D,F) Tg(gata1:dsRed) was used to visualize the blood cells flowing through the glomerular capillaries. (B) Wild-type larvae on the same plane as A show gata1-positive blood cells in the glomerular capillaries (arrows; see supplementary material Movie 1). The bright red line of cells running through the middle of the larvae is the dorsal aorta (arrowhead). (D) Mismatch MO-injected larvae also have blood cells flowing through the glomerular capillaries (arrows; see supplementary material Movie 2) and through the aorta (arrowhead). (F) ponzr1 morphants have no blood cells in the glomerular capillaries, but the cells flowing through the aorta can still be seen (arrowhead; see supplementary material Movie 3). (G) This phenotype is significantly different compared with both wild-type and mismatch MO-injected larvae (^*** P<0.001).

Expression Data

Genes:	DsRed EGFP
Fish:	li1Tg sd2Tg li1Tg + MO1-ponzr1 sd2Tg + MO1-ponzr1
Knockdown Reagent:	MO1-ponzr1
Anatomical Terms:	nucleate erythrocyte pronephric podocyte
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-ponzr1
Knockdown Reagent:	MO1-ponzr1
Observed In:	pronephric glomerular capillary
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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