Fig. 3

Expression of the muscle-specific alternative isoforms of UFD2a is conserved in mice and zebrafish.

A). Among 8 murine tissues, UFD2a-7/7a is expressed only in striated muscle. Protein lysates prepared from adult murine tissues analyzed by immunoblotting using a rabbit polyclonal antibody recognizing UFD2a. B). Schematic diagram depicting the exons of zebrafish ube4B (UFD2a ortholog) and the location of various primer sets used for RT-PCR. C). RNA was extracted from whole 7 pfd zebrafish embryos and subjected to RT-PCR analysis using primers F2 and R2. The 333-bp band represents the fragment expected from the amplification of exons 6, 8, and 9, and the 873-bp fragment includes amplification of the suspected exons 7 and 7a. D). RNA extracted from 4 tissues from multiple adult zebrafish was analyzed by RT-PCR using primers F2 (specific for exon 7) and R2 (exon 8).