FIGURE

Fig. 1

ID

ZDB-FIG-120131-45

Publication

Du et al., 2012 - Differential regulation of epiboly initiation and progression by zebrafish Eomesodermin A

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Fig. 1

eomesa^fh105 mutant allele. Schematic of the 661 amino acid full-length Eomesa protein with T-domain in blue. Location of the stop codon in allele fh105 marked by asterisk. (B) Images of heterozygous (top) and homozygous (bottom) eomesa^fh105 adult fish. (C) Control western blot for the anti-Eomesa antibody, lanes as indicated. (D)Western blot of sphere stage wild type and MZeomesa embryos, lanes as indicated. 15 embryo equivalents loaded per lane. (E) Whole-mount in situ hybridization for eomesa on sphere stage embryos of the indicated genotypes.

Expression Data

Gene:	eomesa
Antibody:	Ab1-eomesa
Fish:	AB eomesa^fh105/fh105 (AB) AB + MO5-eomesa eomesa^fh105/+ (AB)
Knockdown Reagent:	MO5-eomesa
Anatomical Terms:	blastodisc brain whole organism
Stage Range:	Sphere to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	eomesa^fh105/fh105 (AB)
Observed In:	dorsal fin
Stage:	Adult

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 362(1), Du, S., Draper, B.W., Mione, M., Moens, C.B., and Bruce, A.E., Differential regulation of epiboly initiation and progression by zebrafish Eomesodermin A, 11-23, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.