FIGURE

Fig. 8

ID

ZDB-FIG-120126-84

Publication

Neto et al., 2012 - The osr1 and osr2 genes act in the pronephric anlage downstream of retinoic acid signaling and upstream of wnt2b to maintain pectoral fin development

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Fig. 8

Osr gene expression is regulated by retinoic acid signaling. Dorsal views of embryos at the 21- to 24-somite stage after whole-mount in situ hybridization against the genes indicated. Anterior is towards the left. Arrowheads indicate the expression of aldh1a2 (A-D), tbx5a (E-H,L,P) and Osr (I-K,M-O) genes in the anterior somite, developing fin buds and pronephros, respectively. (A-D) aldh1a2 expression is not affected in single or double Osr-morphant embryos. (E-H) Increasing RA signaling is able to rescue tbx5a downregulation caused by DEAB (E-G, arrowheads) but not Osr impairment (H). (I-K,M-O) Reducing (J,N) or increasing (K,O) RA signaling, reduced or expanded, respectively, the expression domain of osr1 but not osr2. (L,P) Osr genes can rescue the loss of tbx5a expression when RA signaling is reduced.

Expression Data

Genes:	aldh1a2 osr1 osr2 tbx5a
Fish:	WT WT + MO1-osr1 WT + MO1-osr2 WT + MO1-osr1 + MO1-osr2
Conditions:	chemical treatment: pharmaceutical chemical treatment: all-trans-retinoic acid chemical treatment: all-trans-retinoic acid
Knockdown Reagents:	MO1-osr1 MO1-osr2
Anatomical Terms:	pectoral fin field pronephros somite anterior region
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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