FIGURE

Fig. 7

ID

ZDB-FIG-111220-34

Publication

Chatterjee et al., 2011 - Conserved And Non-Conserved Enhancers Direct Tissue Specific Transcription In Ancient Germ Layer Specific Developmental Control Genes

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Fig. 7

EMSAs and transgenic assays to detect core-binding motif. (A) Electrophoretic Mobility Shift Assays (EMSA) with 7 overlapping probes spanning the 200 bp non-conserved enhancer. Arrows indicate the two probes that bound protein. (B) EMSAs with 5 bp sliding mutant probes for EM4. The star indicates the probe 5 which did not show binding and the subsequent probe 6 showed weak binding. (C) EMSA with 10 bp mutation probes. Mutations in nucleotides 21-30 (M21-30) leads to complete abrogation of binding. Transgenic assays with wild type 40 bp probe (EM4) (D), mutant probe 5 (E), mutant probe 6 (F) and 10 bp mutant probe (G). The enhancer could drive expression in forebrain (FB) and otic vesicle (OV) at 24 hpf that are domains of expression of otx1b.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.