FIGURE

Fig. 1

ID

ZDB-FIG-111220-11

Publication

Kimmel et al., 2011 - Requirement for Pdx1 in specification of latent endocrine progenitors in zebrafish

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Fig. 1

ins mRNA expression in hb9 and pdx1 morphants. In situ detection of ins mRNA in wild-type embryos (A,B), hb9 morphants (C,D), pdx1 morphants (E,F) and hb9/pdx1 double morphants (G,H) at 24 h post fertilization (hpf) and 5 days post fertilization (dpf). In hb9 and pdx1 morphants the number of ins expressing cells is strongly reduced at 24 hpf as compared to control embryos (A,C,E) but has substantially increased by 5 dpf (D,F). (G,H) ins expression is missing in most double morphants (arrow in H marks a single ins positive cell). Embryos are shown from ventral (24 hpf) and lateral (5 dpf) view with anterior to the right. Scale bars correspond to 20 μm (24 hpf) and 50 μm (5 dpf). (I) Proportion of embryos showing > 5 Ins+ cells in control and morphant embryos at 3 dpf and 5 dpf. Beta cells emerge between 3 dpf and 5 dpf in hb9 and pdx1 morphants, while there is persistent absence in the hb9/pdx1 double morphant.

Expression Data

Gene:	ins
Fish:	WT WT + MO1-pdx1 WT + MO2-mnx1 WT + MO1-pdx1 + MO2-mnx1
Knockdown Reagents:	MO1-pdx1 MO2-mnx1
Anatomical Terms:	endocrine pancreas pancreatic B cell posterior pancreatic bud
Stage Range:	Prim-5 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-pdx1 WT + MO2-mnx1 WT + MO1-pdx1 + MO2-mnx1
Knockdown Reagents:	MO1-pdx1 MO2-mnx1
Observed In:	endocrine pancreas development pancreatic B cell posterior pancreatic bud
Stage Range:	Prim-5 to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Biol.