FIGURE

Fig. 3

ID

ZDB-FIG-111215-45

Publication

Li et al., 2011 - Abca12-mediated lipid transport and Snap29-dependent trafficking of lamellar granules are crucial for epidermal morphogenesis in a zebrafish model of ichthyosis

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Fig. 3

Knockdown of abca12 and snap29 expression by morpholinos. (A) Knockdown of abca12. (B) Knockdown of snap29. MO1 and MO2 morpholinos (right lanes, the upper panel), which target the splice donor site at the exon-4–intron-4 border of the corresponding genes, prevents pre-mRNA splicing. The consequences of MO1 on abca12 pre-mRNA splicing and MO2 on snap29 mRNA splicing were determined by RT-PCR. The results showed the retention of intron 4 in the majority of mRNA transcripts (>90%) as compared with the normally transcribed control. The mRNA levels were normalized by the level of β-actin mRNA (lower panels). cDNA and gDNA represent amplification of the corresponding complementary DNA and genomic DNA, respectively. Injections with the 5-bp mismatched control morpholino for abca12 (cMO) or global standard control morpholino (scMO) did not alter pre-mRNA processing, similar to the uninjected controls or those injected with phenol red.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.