FIGURE

Fig. 6

ID

ZDB-FIG-111215-39

Publication

Phillips et al., 2011 - Harmonin (Ush1c) is required in zebrafish Muller glial cells for photoreceptor synaptic development and function

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Harmonin localizes in Müller cells through all zebrafish life stages and is evolutionarily conserved. (A–F) Localization of anti-zebrafish harmonin (red) in Müller cells persists in adult zebrafish retinas. Some low level signal is sometimes observed in photoreceptor synapses (arrow in A) and in photoreceptor outer segments (arrow in D). The strongest signal within the photoreceptor layer is associated with Müller cell processes projecting into the subapical region (asterisks). Nonspecific fluorescence of photoreceptor outer segments at this stage can be compared with panel H in supplementary material Fig. S4. (G–L) Anti-mouse harmonin (SDI; G,I) and anti-human harmonin (Santa Cruz; J,L) detect localization in photoreceptors and Müller cells [glutamine synthetase (GS) is labeled in green; harmonin antibodies in red] in retinas from P10–P11 mouse pups. The outer nuclear layer (onl), inner nuclear layer (inl) and ganglion cell layer (gcl) are labeled in merged panels. Scale bars: 50 μm (A–C,G–L); 20 μm (D–F).

Expression Data

Gene:	ush1c
Antibodies:	Ab1-glul Ab1-ush1c
Fish:	AB
Anatomical Terms:	Muller cell Muller cell cell projection
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.