Fig. S3
Expression patterns of TgBAC(pax3a:GFP)i150 A-C: Lateral view of a 24hpf live embryo showing strong expression in the diencephalon, mid-hindbrain, dorsal spinal cord, cranial neural crest cells and trunk neural crest.D:Cross-section of a 24hpf embryo stained with F310 to mark the fast muscle and smhc in situ probe to mark the slow muscle. Expression can be seen in the dorsal spinal cord, trunk neural crest and dermomyotome. E,F: Lateral views of 24hpf embryos, whereby E is more superficial than F. G: Lateral view of a 24hpf embryo stained with F310 to mark the fast muscle and smhc in situ probe to stain the slow muscle. Trunk neural crest cells can be seen. H: Dorsal view of 48hpf embryo with strong expression in the mid-hindbrain and in the pectoral fins. I: Lateral view of a 48hpf embryo shows next to strong brain expression many neural crests on the eye. J: At 48hpf, the expression is seen in the trunk neural crest cells and dorsal spinal cord. K: A lateral image stack at 72hpf reveals expression in cranial ganglia, which lies medial to the eye. L: At 72hpf, processes of spinal cord neurons can be seen. M: At 72hpf, expression is seen in multiple trunk crest cells and myotomal cells. N: At 4dpf a cell with processes is seen on the yolk ball. O: At 4dpf, mutltiple Pax3a:GFP cells are in the caudal fin. P: Pax3a:GFP expression can also be found in the pectoral fin at 4dpf. Q: Pax3a:GFP continues to be expressed within the myotome. R: At 5dpf, Pax3a:GFP expression is in the dorsal spinal cord. S: At 6dpf, numerous Pax3a:GFP cells can be found elongated alongside myofibres as well as rounded up and some along the myosepta. T-V: Lateral view of a 6dpf fish with strong expression in the mid-hindbrain, dorsal spinal cord, myotome, and pectoral fin. W: At 10dpf, Pax3a:GFP can be seen in many cells in the trunk region. |