FIGURE

Fig. 2

ID

ZDB-FIG-111102-2

Publication

Gutnick et al., 2011 - The hypothalamic neuropeptide oxytocin is required for formation of the neurovascular interface of the pituitary

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Fig. 2

Axovasal Interactions in the Zebrafish Pituitary

(A–D) Three-dimensional reconstructions of the hypophysis of 9-day-old zebrafish embryos carrying pairs of transgenic fluorescent markers of different hypophyseal components.

(A) The oxtl:EGFP transgene (green) marks oxytocinergic axons terminating at the neurohypophysis, just dorsal and posterior to the adenohypophyseal prolactin-producing cells marked with prl:RFP (red).

(B) Double transgenic animals expressing oxtl:EGFP (green) together with the vascular endothelial marker vegfr2:Cherry (red) reveal a distinct, previously unidentified structure of the zebrafish hypophyseal vasculature.

(C and D) Hypophyseal vasculature, visualized by either vegfr2:EGFP or vegfr2:Cherry, together with the adenohypophyseal markers prl:RFP and pomc:EGFP. The ring-like vascular structure resided dorsal to the adenohypophysis. (B2) and (D2) show optical Z-slices that demonstrate the position of the NH in an indentation of the adenohypophysis.

(E) Schematic map of ventral head vasculature of the zebrafish larvae, including the location of the hypophyseal vasculature. The asterisk marks the location of the neurohypophysis.

AH, adenohypophysis; CrDI, cranial division of the internal carotid artery; NH, neurohypophysis; OA, optic artery; PLA, palatocerebral artery; PLV, palatocerebral vein; pomc, proopiomelanocortin; prl, prolactin; vegfr, vascular endothelial growth factor receptor. Scale bar, 20 μm. See also mmc3VIDEO, mmc4VIDEO and mmc5VIDEO.

Expression Data

Genes:	EGFP GFP mCherry RFP
Fish:	s843Tg; zf113Tg s896Tg; wz01Tg s896Tg; zf44Tg wz01Tg; zf113Tg
Anatomical Terms:	adrenocorticotropic hormone secreting cell hypophysis vascular endothelium neurohypophysis axon terminus prolactin secreting cell
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 21(4), Gutnick, A., Blechman, J., Kaslin, J., Herwig, L., Belting, H.G., Affolter, M., Bonkowsky, J.L., and Levkowitz, G., The hypothalamic neuropeptide oxytocin is required for formation of the neurovascular interface of the pituitary, 642-654, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell