FIGURE

Fig. S1

ID

ZDB-FIG-111018-10

Publication

Patra et al., 2011 - Nephronectin regulates atrioventricular canal differentiation via Bmp4-Has2 signaling in zebrafish

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Fig. S1

Characterization of splice-inhibitory morpholino MO3-mediated Npnt knockdown. (A) Scheme of the splice-inhibitory effect of MO3. npnt exon E3 could not be detected by RT-PCR. (B) RT-PCR analysis of mRNAs from control and MO3-injected embryos at 52 hpf. (C) Western blot analysis of protein extracts from control and MO3-injected embryos at 52 hpf. (D) Lateral view of control and MO3-injected embryos at 75 hpf. MO3 injection resulted in pericardial edema (arrowhead). (E) MO3-injected embryos from transgenic Tg(myl7:EGFP-HsHRAS)^s883 zebrafish showed an expansion of the AV canal at 52 hpf. Brackets indicate the AV boundary. A, atrium; V, ventricle. Scale bars: 500 μm in D; 50 μm in E.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-npnta s883Tg + MO3-npnta
Knockdown Reagent:	MO3-npnta
Observed In:	atrioventricular canal pericardium
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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