FIGURE

Fig. 3

ID

ZDB-FIG-111017-7

Publication

Ramachandran et al., 2011 - Ascl1a/Dkk/ beta -catenin signaling pathway is necessary and glycogen synthase kinase-3 beta inhibition is sufficient for zebrafish retina regeneration

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Fig. 3

β-Catenin accumulation in MG-derived progenitors is necessary for retina regeneration. (A and B) Injury-induced β-catenin expression at 4 dpi is blocked by Dkk1b overexpression in hs:Dkk1GFP fish. (C) Injury-dependent induction of GFP expression in the β-catenin reporter fish, TOPdGFP. (D) Accumulation of β-catenin in GFP⁺ MG-derived proliferating progenitors of 1016 tuba1a:gfp fish at 4 dpi. (Upper) Standard immunohistochemical protocol. (Lower) Epitope retrieval protocol. (E and F) Pyrvinium and XAV939 inhibit the generation of GFP⁺, β-catenin⁺, and BrdU⁺ MG-derived progenitors at 4 dpi in 1016 tuba1a:gfp fish. White dot identifies an area of autofluorescence that results from long exposures to detect fluorescent signals. *P < 0.001. (Scale bars, 10 μm.) Abbreviations are as in Fig. 1.

Expression Data

Gene:	GFP
Antibody:	Ab2-ctnnb
Fish:	WT mi4Tg w25Tg w32Tg
Conditions:	heat shock, physical alteration: anatomical structure chemical treatment: pyrvinium, physical alteration: anatomical structure physical alteration: anatomical structure chemical treatment: pharmaceutical, physical alteration: anatomical structure
Anatomical Terms:	Muller cell retinal ganglion cell layer retinal inner nuclear layer retinal outer nuclear layer
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA