Fig. 4

Comparison of wild-type, han^s6, han^c99 and transheterozygote phenotypes. (A-O) Dorsal views, anterior to the top. (A,E,I,M,P) Wild-type embryos; (B,F,J,N,Q) han^c99 mutants; (C,G,K) transheterozygous mutants; (D,H,L,O,R) han^s6 mutants. In all cases, the han^s6 phenotype is most severe, the han^c99 phenotype is most mild and the transheterozygote phenotype is intermediate. (A-D) cmlc2 expression at 24 hpf; (E-H) tbx5 expression at 24 hpf. Arrowheads (F,G) indicate myocardial tissue. (I-L) gata4 expression at the 16-somite stage. (M-O) cmlc2 expression at the 16-somite stage. (P-R) Lateral views of pectoral fin buds, anterior to the left, demonstrating expression of dlx2 in the apical epidermal fold (arrowheads) at 48 hpf. Continuous and strong dlx2 expression is observed in the apical fold of wild-type pectoral fins (P) (Akimenko et al., 1994), but dlx2 maintenance is defective in han^c99 (Q) and han^s6 (R) mutants.