FIGURE

Fig. S3

ID

ZDB-FIG-111011-2

Publication

Bussmann et al., 2011 - Rapid BAC selection for tol2-mediated transgenesis in zebrafish

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Fig. S3

Comparison of transgenic reporter mRNA expression with endogenous mRNA expression. Whole-mount in situ hybridization with antisense RNA probes to (A) cdh5 or (B) gfp/yfp in Tg(cdh5:gal4)^mu101; Tg(uas:gfp)^nkuasgfp1a double-transgenic embryos (Bussmann et al., 2011) and to (C) cxcl12b or (D) gfp/yfp in Tg(cxcl12b:yfp)^mu102 transgenic embryos. The expression patterns of the transgenic reporter construct and the endogenous gene were found to be identical. We note that the expression levels of the cxcl12b:yfp reporter were similar to those of the endogenous cxcl12b gene, whereas the expression levels of the cdh5:gal4, uas:gfp reporter were significantly higher than those of the endogenous cdh5 gene. No expression differences of the endogenous genes were detected in transgenic versus non-transgenic embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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