Chd7 morpholino (MO) injection results in a gradient of phenotypic defects in developing zebrafish. A: Representatives of the classes of chd7 morphant phenotypes at 48 hours postfertilization (hpf). The top embryo is a control MO injected embryo. The remaining embryos represent the mild, moderate, and severe classes of chd7 morphants. B: Graph showing the percentage of embryos in each morphant phenotype category upon injection with 6 ng/nl of chd7 translation blocking, chd7 splice blocking, standard control, or chd7 5 base pair mismatch control morpholino. C: Reverse transcriptase-polymerase chain reaction (RT-PCR) exhibiting the loss of proper splicing of chd7 mRNA in chd7 splice blocking morphants. The increased band size is an indicator of the retention of chd7 intron 2 following mRNA processing. D: A green fluorescent protein (GFP) mRNA injected embryo illustrating the even distribution of the injected material throughout the animal. C, control morpholino; SB, splice blocking morpholino; WT, wild-type.
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