FIGURE

Fig. 10

ID

ZDB-FIG-110929-26

Publication

Greenhill et al., 2011 - An iterative genetic and dynamical modelling approach identifies novel features of the gene regulatory network underlying melanocyte development

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Fig. 10

Mitfa-dependent maintenance of mitfa expression.

A,B) Expression of Mitfa is reduced in mitfa^b692 mutant. A, B) Embryos from incross of mitfa heterozygotes were treated with PTU and processed for in situ hybridization with mitfa probes at 30 hpf stage. A majority (53/69; 73%) showed normal strong mitfa expression and were presumed wild-type siblings (A, WT), whereas 33/124 (27%) had weakened expression and were presumed mitfa mutants (B). C–J) Injection of RNA encoding WT Mitfa drives ectopic expression of dct (C,G) and mitfa (D,H) at both 6 hpf (C,D) and 10.5 hpf (G,H), whereas RNA encoding the Mitfa(b692) mutant form does not (E,F,I,J). Expression of the endogenous mitfa gene is detected using an anti-sense probe corresponding to the 32 UTR of the gene, a sequence absent from the injected RNA. Scale bar:100 μm.

Expression Data

Gene:	mitfa
Fish:	WT mitfa^b692/b692
Condition:	chemical treatment: N-phenylthiourea
Anatomical Term:	melanocyte
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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