FIGURE

Fig. S2

ID

ZDB-FIG-110928-30

Publication

Culbertson et al., 2011 - Chondrogenic and Gliogenic Subpopulations of Neural Crest Play Distinct Roles during the Assembly of Epibranchial Ganglia

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Fig. S2

Endodermal pouches are present in NC-depleted mutants. (A–D) Ventral views showing nkx2.3 expression as detected by in situ hybridization in the head of wildtype (A) and foxd3 (B), sox10 (C) and prdm1a (D) mutants at 2 dpf. Endodermal pouches expressing nkx2.3 transcript are visible as bilateral sets of parallel linear expression domains posterior to the eyes (A, brackets). (E and F) Lateral view of Zn-5 immunofluorescence (red) marking endodermal pouches at 5 dpf in wildtype (E, arrowheads) and disc-1 morphant (F). Embryos were stained with DAPI (blue) to visualize nuclei. Scale bar in (A) = 100 μm. Scale bar in (E) = 50 μm.

Expression Data

Gene:	nkx2.3
Antibody:	zn-5
Fish:	WT foxd3^zdf10/zdf10 prdm1a^nl3/nl3 sox10^t3/t3 WT + MO3-disc1
Knockdown Reagent:	MO3-disc1
Anatomical Term:	pharyngeal pouch
Stage Range:	Long-pec to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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