FIGURE

Fig. S1

ID

ZDB-FIG-110909-36

Publication

Johnson et al., 2011 - Vgll2a is required for neural crest cell survival during zebrafish craniofacial development

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Fig. S1

Injection with vgll2a e2i2 MO results in reduced vgll2a message and defects of the pharyngeal skeleton. A representative example of an uninjected embryo (A) and an embryo injected with 10 ng vgll2a e2i2 MO stained with alcian blue at 5 dpf in a lateral view (B) and RT-PCR of the vgll2a message in these embryos (C). Injection with 10 ng vgll2a e2i2 MO resulted in hypoplasia or absence of the pharyngeal cartilages as stained with alcian blue (B, n = 6/6), similar to the phenotype observed upon injection with the vgll2a ATG MO (Fig. 2D). RT-PCR of the entire vgll2a coding sequence (C) shows 10 ng vgll2a e2i2 MO results in reduced vgll2a message (44% reduction as measured by densitometry). Anterior is to the left in A and B.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-vgll2a
Knockdown Reagent:	MO1-vgll2a
Observed In:	pharyngeal arch cartilage
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 357(1), Johnson, C.W., Hernandez-Lagunas, L., Feng, W., Melvin, V.S., Williams, T., and Artinger, K.B., Vgll2a is required for neural crest cell survival during zebrafish craniofacial development, 269-81, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.