FIGURE

Fig. 6

ID

ZDB-FIG-110622-90

Publication

Yang et al., 2011 - BMP and non-canonical Wnt signaling are required for inhibition of secondary tail formation in zebrafish

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Fig. 6

Defects of the caudal end of notochord in mfn and kny initiate during tailbud protrusion. (A) Dorsal view of flat-mounted tailbud of a 6-somite wild-type zebrafish embryo, after fluorescence in situ hybridization (FISH) for flh (red) and antibody staining for P-Samd5 (green); no colocalization (merge) is seen. (B,C) Lateral view of tails of 14-somite wild-type and mfn embryos exposed to P-Smad5 (green) and β-catenin (red) antibodies. P-Smad5 staining is absent in the posterior tailbud of mfn (C, merge). Arrowheads indicate the ventroposterior cells in the tailbud that are normally positive for P-Smad5 (B, merge). (D-F4) Confocal time-lapse recording of tailbuds in embryos expressing Tg(flh:EGFP) transgene (green) and mRFP (red) from the 10-somite stage. Shown are wild type (D-D4) and mfn (E-E4) and kny (F-F4) mutants. Note that transgenic EGFP is retained in the caudal notochord, floorplate and periphery of Kupffer′s vesicle (KV; marked by the white line). The caudal end of notochord (CN) is indicated by blue line. Arrowheads mark the ventroposterior cells in the tailbud. Embryos were all mounted laterally.

Expression Data

Genes:	EGFP noto
Antibodies:	Ab1-smad Ab2-ctnnb
Fish:	WT tll1^{tc263a/tc263a} c161Tg gpc4^fr6/+; c161Tg tll1^tc263a/+; c161Tg
Anatomical Terms:	posterior neural plate tail bud
Stage Range:	5-9 somites to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tll1^{tc263a/tc263a} c161Tg gpc4^fr6/+; c161Tg tll1^tc263a/+; c161Tg
Observed In:	notochord formation post-anal tail morphogenesis post-vent region
Stage Range:	10-13 somites to 14-19 somites

Phenotype Detail

Acknowledgments

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