Fig. 2

cbx7a and cbx7b are expressed in zebrafish larvae. (A) Schematic representation of zebrafish cbx7a and cbx7b genes and proteins. On the gene organization cartoons, white parts indicate untranslated regions whereas red parts correspond to regions coding for the Pc boxes. On the protein drawings, the chromodomain is indicated as a blue triangle and the Pc box as a red circle. Amino acid length of the proteins is indicated. Position of primers used for the RT-PCR is shown as green arrows. (B) Detection of cbx7a and cbx7b transcripts in zebrafish larvae by RT-PCR. Total RNAs were extracted from 5 dpf zebrafish larvae and subjected to reverse transcription and PCR amplification using primers specific for cbx7a and cbx7b, as indicated in (A), in presence (+ RT) or absence (- RT) of reverse transcriptase. The expected size of the PCR products is 243 and 125 bp for cbx7a and cbx7b, respectively.