FIGURE

Fig. 4

ID

ZDB-FIG-110527-31

Publication

Hong et al., 2011 - The transcriptional mediator component med12 is required for hindbrain boundary formation

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Fig. 4

Cell proliferation defects in the hindbrain of kto^y82 mutants.

All images are dorsal views at 19 hpf (C,D), 26 hpf (A,B), and 28 hpf (E,F,H,I). (A,B) Analysis of cell death using TUNEL assay. (C–G) PH3 staining (C–F), and quantification (G) of PH3 positive cells in wt and kto embryos. (H–J) BrdU staining of wt and mutant embryos (H,I), and quantification of BrdU positive cells (J). Five embryos were counted for each condition; the error bars indicate 1 standard deviation based on 5 samples. PH3 staining at 19 hpf, wt vs. kto: p = 0.003456; at 28 hpf, wt vs. kto: p = 2.97e-05. BrdU staining, wt vs. kto: p = 0.000706. ov, otic vesicle.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	med12^y82/y82
Observed In:	apoptotic process cell proliferation in hindbrain
Stage Range:	20-25 somites to Prim-5

Phenotype Detail

Acknowledgments

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