FIGURE

Fig. 3

ID

ZDB-FIG-110527-3

Publication

Krueger et al., 2011 - Flt1 acts as a negative regulator of tip cell formation and branching morphogenesis in the zebrafish embryo

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Fig. 3

Flt1 regulates endothelial tip cell formation. (A,B) Time-lapse in vivo imaging shows increased tip cell numbers and proliferating endothelial tip cells in segmental vessels of zebrafish flt1 morphants. Note that in flt1 morphants the leading cell number 1 gives rise to two progeny cells termed 1.1 and 1.2. y-axis shows the percentage of examined flt1 morphants with proliferation in tip or stalk cells. (C) Quantification of endothelial cell numbers in four consecutive segmental vessel (ISV) – dorsal longitudinal anastomotic vessel (DLAV) loops reveals an increase in flt1 morphants. (D) Anti-phospho-histone H3 staining confirms endothelial proliferation in segmental vessels of flt1 morphants. **, P<0.01; Student′s t-test. Error bars indicate s.e.m.

Expression Data

Gene:	EGFP
Fish:	y1Tg y1Tg + MO3-flt1
Knockdown Reagent:	MO3-flt1
Anatomical Terms:	dorsal aorta dorsal longitudinal anastomotic vessel intersegmental vessel
Stage Range:	Prim-15 to High-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg + MO3-flt1
Knockdown Reagent:	MO3-flt1
Observed In:	blood vessel endothelial cell blood vessel morphogenesis endothelial tip cell intersegmental vessel sprouting angiogenesis vascular sprouts
Stage Range:	Prim-15 to High-pec

Phenotype Detail

Acknowledgments

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