FIGURE

Fig. s2

ID

ZDB-FIG-110517-6

Publication

Hicken et al., 2011 - Sublethal exposure to crude oil during embryonic development alters cardiac morphology and reduces aerobic capacity in adult fish

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Fig. s2

CYP1A immunofluorescence in embryos exposed to gravel effluents. Embryos were exposed from 4 to 48 hpf in either clean (A) or oiled-gravel effluent (B–F), fixed and processed for whole-mount CYP1A (green) and myosin heavy chain (red) immunofluorescence as described under Materials and Methods. The myosin heavy chain antibody recognizes both skeletal and cardiac muscle and serves as an internal control for permeabilization. Images in A and B are epifluorescent, C and D are confocal stack projections, and E and F are confocal optical sections. Embryos from clutch 2 exposed to clean (A) or oiled (B) gravel effluent. (C and D) CYP1A immunofluorescence in the epidermal cells on the head of embryos from clutch 2 and clutch 3, respectively. Eye and pectoral fins (pf) are indicated. (E and F) CYP1A immunofluorescence in endocardial cells (arrowheads) of the cardiac atrium (a) and ventricle (v), and epithelium (arrows) over the pericardial region. (Scale bars: 100 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA