Nav3a is expressed during liver development. (A) Nav3a contains a calponin-homology domain, an LKK-actin binding domain, a putative SH3 domain and ATPase domains of the AAA type. (B) Nav3a is highly related to Nav3 members of other species. (C) Whole-mount in situ hybridization for nav3a in zebrafish embryos. The boxed areas in the upper panels are presented at higher magnification in the lower panels. At 22 hpf, nav3a expression could be detected predominantly in the intestinal endoderm (arrowhead, left panel). Between 26 and 40 hpf, endodermal nav3a expression expands to the newly formed liver bud (arrowhead, middle and right panel). (D) Expression of nav3a and the early liver markers hhex and prox1 analyzed by in situ hybridization in zebrafish embryos at 22 hpf. Nav3a expression could be detected in the foregut endoderm (arrowhead, left panel). hhex and prox1 expression is limited to pancreatic precursors but is not detectable in the presumptive liver bud region (arrowhead, middle and right panel). C and D show dorsal views.
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