FIGURE

Fig. 3

ID

ZDB-FIG-110407-24

Publication

Wühr M. et al., 2010 - A Model for Cleavage Plane Determination in Early Amphibian and Fish Embryos

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Fig. 3

Aster Movement Depends on Dynein-Dependent Pulling Forces

(A) Structure of caged combretastatin 4A.

(B) EMTB-3GFP zebrafish embryos were incubated in caged combretastatin and subjected to UV irradiation in defined region (marked in red). Within seconds, microtubules depolymerized selectively on the irradiated side. The remaining aster moved away from the irradiated region, arguing for pulling forces on asters. Full arrows mark the positions of centrosomes; hollow arrows on right mark their original positions.

(C) Injection of p150-CC1 blocks aster movement. Asters still grew out and broke down under cell-cycle control but lost their ability to move or orient centrosomes. See also Figure S3 and Movies S3 and S4.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.