FIGURE

Fig. S3

ID

ZDB-FIG-110218-9

Publication

Barrios et al., 2003 - Eph/Ephrin signaling regulates the mesenchymal-to-epithelial transition of the paraxial mesoderm during somite morphogenesis

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Fig. S3

Epithelialization of Host Cells May Be Independent of Ephrin Reverse Signaling and Independent of Cell-Nonautonomous Activity of Papc
(A–D) Confocal images showing Bodipy 505-515-labeled somitic mesoderm of fss^-/- host embryos (green) containing rhodamine dextranlabeled donor cells (red). Donor cells are wild-type cells expressing (A) EphA4, (B) fss^-/- cells expressing EphA4 and Papc, (C) wild-type cells expressing EphA4 and dominant-negative Papc, or (D) wild-type cells expressing EphA4 and containing Papc morpholino. In (A), fss^-/- host cells are expressing truncated, dominant-negative Ephrin-B2a-eGFP. The arrows point to basally localized nuclei of fss^-/- host cells at boundaries created between donor and host cells. The scale bar represents 5 μm.
(E) Summary of experiments investigating the role of Ephrin-B2a and Papc upon epithelialization.
(F and G) Controls for the use of the papc morpholino (see the Supplemental Experimental Procedures).
(H and I) Dorsal views of somitic mesoderm of a wild-type embryo and an embryo injected with papc morpholino. Somite boundaries are not properly formed, and myoD expression is downregulated in the somites of Papc morphant embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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