FIGURE

Fig. S5

ID

ZDB-FIG-110105-23

Publication

Labalette et al., 2011 - Hindbrain patterning requires fine-tuning of early krox20 transcription by Sprouty 4

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Fig. S5

Enhanced FGF signalling in Spry4 morphants. (A) Enhanced ERK1/2 phosphorylation in Spry4 morphants. Control and Spry4mo-injected embryos were collected at 100% epiboly and subjected to western blot analysis using antibodies against phosphoERK1/2 (pERK1/2) or total ERK1/2. Actin was used as a loading control. (B-E) Immunostaining for pERK1/2 (B,C) and in situ hybridisation for pea3 (D,E) of Ctrlmo- and Spry4mo-injected embryos at 100% epiboly. Embryos are shown as dorsal views with anterior to the top. The insets show tailbud views, allowing determination of the developmental stage by evaluation of the closure of the tailbud. tb, tailbud; hb, hindbrain; tl, telencephalon.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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