FIGURE

Fig. S2

ID

ZDB-FIG-101223-30

Publication

Lee et al., 2010 - The habenula prevents helpless behavior in larval zebrafish

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Fig. S2

Related to Figure 3.

(A-C) Dorsal view of the habenula of a KR11 fish, irradiated after injection of FITC-annexin V into the forebrain. The images show relative change in FITC fluorescence intensity (F/F₀) following irradiation with green light.

(A) Prior to irradiation.

(B) After 2 minutes of irradiation.

(D) The habenula of a nontransgenic fish that had been injected with FITC-annexin V, then irradiated for 4 minutes. There is no signal in habenula afferents. Image processing in panels A-D was carried out using the ?F div F0? plugin in imageJ. A Gaussian filter (sigma = 2.0) was used after normalization.

(E) Dorsal view of a KR4 transgenic fish. Cells in the circumventricular organ, anterior to the habenula, as well as the parapineal organ, express KillerRed. The region bounded by the white circle was irradiated. A similar region was irradiated in KR11 fish used in behavioral experiments.

Pa: pallium; OT: optic tectum; rHb: right habenula; lHb: left habenula. Anterior is to the left.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.