FIGURE

Fig. S1

ID

ZDB-FIG-101206-29

Publication

Seth et al., 2010 - Core fucosylation is required for midline patterning during zebrafish development

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Fig. S1

Supporting Figure S1: RT-PCR analysis indicates that FucT8 morpholino oligonucleotides were effective in blocking processing of FucT8 mRNA. RNA extracted from 24-hpf control and FucT8 morphant embryos was subjected to RT-PCR using two primers pairs; the first pair (green, red arrows) produced a 270-bp fragment (arrow) in FucT8 morphants, that is not present in control-injected embryos. The second primer pair used the same forward primer (green) along with a reverse primer (blue) that generated a 266-bp product in control embryos, as well as a faint, larger PCR product of 2,858 bp (arrow) in FucT8 morphants that is not easily reproduced in the figure, as the PCR reaction was optimized for smaller reaction products.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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